your trusted partner for assessing genetic status of cell lines
FACT
Actively proliferating cells are prone to acquire genetic changes, scaling from single nucleotide variants to large chromosomal aberrations. This phenomenon corresponds to any cells in culture and is highly discussed by the stem cell research community and ATMP network.
CHALLENGE
Regular monitoring of the genetic status of cell cultures is required to prove the validity of any data collected from the cell culture model system. Therefore, an appropriate genetic testing strategy is crucial for every project based on cultured cells.
SOLUTION
SoboLAB offers customized genetic testing to verify the genetic status of cell cultures. We work with any type of proliferating cells. The most common requests are for human pluripotent stem cells (hPSC), CAR-T cells, and fibroblasts.
We provide accurate and reliable genetic testing results to ensure the quality of your cell lines.
We keep fast turnaround times for testing your cell lines to ensure your efficiency and productivity.
We offer expert guidance to help you understand the results and take necessary actions.
Chromosomal analysis (karyotyping) is a golden-standard method for evaluation of genetic status of cells. International Society for Stem Cell Research (ISSCR) state karyotyping as a mandatory test for basic characterization of the cell model systems.
Karyotyping is a laboratory technique used to analyze number and structure of chromosomes and detect eventual chromosomal abnormalities. The procedure involves obtaining andculturing the cells before preparation, staining and analyzing the chromosomes under a microscope.
We offer three levels of karyotyping service for different customer needs with turnaround time between 10-20 business days. Our test reports include detailed overview of test results, interpretation and reccomedations for eventual actions.
Our expert consultant provides personalized guidance on genetic testing options, assisting you in selecting and implementing the most effective solutions tailored to your needs.
We help you with genome instability and mutagenesis tests, gene expression analysis, cell proliferation and apoptosis assays.
Maria Sobol has an extensive professional background in clinical cytogenomics and stem cell biology.
Maria has worked with human cytogenetics (karyotyping) since 2004. Starting from 2015, she has combined conventional cytogenetic approaches with molecular assays, like chromosomal microarray, for evaluation of genome status in human cells.
Maria's professional mission is to provide a reliable and customized cytogenomic service to research and GMP facilities.
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PUBLICATIONS
2015
Methods of Reprogramming to Induced Pluripotent Stem Cell
Associated with Chromosomal Integrity and Delineation of a Chromosome 5q Candidate Region for Growth Advantage
Sobol M, Raykova D, Cavelier L, Khalfallah A, Schuster J, Dahl N
Stem Cells and Development
2019
Generation of three human induced pluripotent stem cell (iPSC) lines from three patients with Dravet syndrome carrying distinct SCN1A gene mutations
Schuster J, Fatima A, Sobol M, Norradin F H, Laan L, Dahl N
Stem Cell Research
2016
Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
Pijuan-Galitó S, Tamm C, Schuster J, Sobol M, Forsberg L, Merry C L R, Anneren C
Nature Communication
2020
Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9
Fatima A, Schuster J, Akram T, Sobol M, Hoeber J, Dahl N
Stem Cell Research
2019
Transcriptome and Proteome Profiling of Neural Induced Pluripotent Stem Cells from Individuals with Down Syndrome Disclose Dynamic Dysregulations of Key Pathways and Cellular Functions
Sobol M, Klar J, Laan L, Shahsavani M, Schuster J, Anneren G, et al.
Molecular Neurobiology
2021
Syndromic RNA polymerase II insufficiency: Generation of a human induced pluripotent stem cell line (UUIGPi002A-5) with a heterozygous disruption of POLR2A
Schuster J, de Guidi C, Fatima A, Sobol M, Dahl N
Stem Cell Research
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